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Quantitative Human ACP2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX224

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Specifications
Product Description
The quantitative human ACP2 (glycosylated) sandwich ELISA kit is designed to detect human acid phosphatase 2 (ACP2) levels. ACP2 is a lysosomal enzyme involved in the hydrolysis of phosphate esters, playing a role in bone resorption and lysosomal function. Its activity is implicated in certain metabolic and bone diseases. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 25.024 pg/mL, which can accurately detect low concentrations of ACP2 in the sample.
Target
ACP2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
25.024 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
65 pg/mL-3500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Acid phosphatase 2
Alternate Names
ACP2; Acid phosphatase 2
Uniprot No.
N-Glycosylation Site
92, 133, 167, 177, 191, 267, 322, 331
Application Notes
Application
The quantitative human ACP2 (glycosylated) sandwich ELISA kit is valuable in research investigating bone metabolism, lysosomal storage diseases, and certain cancers. Researchers utilize this ELISA to quantify ACP2 levels, providing insights into its role in these conditions and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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