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Quantitative Human ARSB (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX254

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Specifications
Product Description
The quantitative human ARSB (glycosylated) sandwich ELISA kit is designed to detect human arylsulfatase B (ARSB) levels. ARSB is an enzyme that breaks down mucopolysaccharides. This protein is found in lysosomes, which plays a role in the degradation of dermatan sulfate and chondroitin sulfate. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.064 ng/mL, which can accurately detect low concentrations of ARSB in the sample.
Target
ARSB
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.064 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Arylsulfatase B
Alternate Names
ARSB; Arylsulfatase B; ASB
Uniprot No.
N-Glycosylation Site
188, 279, 291, 366, 426, 458
Application Notes
Application
The quantitative human ARSB (glycosylated) sandwich ELISA kit is designed to measure ARSB levels in biological samples. Researchers employ it in studies investigating lysosomal storage disorders, such as Maroteaux-Lamy syndrome. The kit is a valuable tool in genetic research and the development of enzyme replacement therapies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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