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Quantitative Human ARSB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX254)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ARSB (glycosylated) sandwich ELISA kit is designed to detect human arylsulfatase B (ARSB) levels. ARSB is an enzyme that breaks down mucopolysaccharides. This protein is found in lysosomes, which plays a role in the degradation of dermatan sulfate and chondroitin sulfate. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.064 ng/mL, which can accurately detect low concentrations of ARSB in the sample.
Target ARSB
N-Glycosylation Site 188, 279, 291, 366, 426, 458
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.064 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Arylsulfatase B
Alternate Names ARSB; Arylsulfatase B; ASB
Uniprot No. P15848
Application The quantitative human ARSB (glycosylated) sandwich ELISA kit is designed to measure ARSB levels in biological samples. Researchers employ it in studies investigating lysosomal storage disorders, such as Maroteaux-Lamy syndrome. The kit is a valuable tool in genetic research and the development of enzyme replacement therapies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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