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Quantitative Human BDH1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX520)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human BDH1 (glycosylated) sandwich ELISA kit is designed to detect human 3-hydroxybutyrate dehydrogenase 1 (BDH1) levels. BDH1 is an enzyme that catalyzes the interconversion of acetoacetate and 3-hydroxybutyrate. These are ketone bodies, which serve as an alternative energy source for the brain and other tissues during periods of fasting or starvation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.079 ng/mL, which can accurately detect low concentrations of BDH1 in the sample.
Target BDH1
O-Glycosylation Site 219
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.079 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name 3-Hydroxybutyrate dehydrogenase 1
Alternate Names BDH1; 3-Hydroxybutyrate dehydrogenase 1; DBH; SDR9C1
Uniprot No. Q02338
Application The quantitative human BDH1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of ketone body metabolism. Researchers use it to measure BDH1 levels in biological samples. The kit has applications in studies of metabolic disorders, nutritional research, and the development of therapies related to energy metabolism.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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