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Quantitative Human BDH1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX520

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Specifications
Product Description
The quantitative human BDH1 (glycosylated) sandwich ELISA kit is designed to detect human 3-hydroxybutyrate dehydrogenase 1 (BDH1) levels. BDH1 is an enzyme that catalyzes the interconversion of acetoacetate and 3-hydroxybutyrate. These are ketone bodies, which serve as an alternative energy source for the brain and other tissues during periods of fasting or starvation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.079 ng/mL, which can accurately detect low concentrations of BDH1 in the sample.
Target
BDH1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.079 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
3-Hydroxybutyrate dehydrogenase 1
Alternate Names
BDH1; 3-Hydroxybutyrate dehydrogenase 1; DBH; SDR9C1
Uniprot No.
O-Glycosylation Site
219
Application Notes
Application
The quantitative human BDH1 (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of ketone body metabolism. Researchers use it to measure BDH1 levels in biological samples. The kit has applications in studies of metabolic disorders, nutritional research, and the development of therapies related to energy metabolism.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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