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Quantitative Human BNP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX264

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Specifications
Product Description
The quantitative human BNP (glycosylated) sandwich ELISA kit is designed to detect human brain natriuretic peptide (BNP) levels. BNP is a hormone that is secreted by the heart. This protein plays a role in regulating blood pressure and fluid balance. It is used as a biomarker for heart failure. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, and plasma. Its sensitivity is 1.735 pg/mL, which can accurately detect low concentrations of BNP in the sample.
Target
BNP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
1.735 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-2000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Brain natriuretic peptide
Alternate Names
BNP; Brain natriuretic peptide; B-Type natriuretic peptide; Ventricular natriuretic peptide
Uniprot No.
O-Glycosylation Site
41, 62, 63, 70, 74, 79, 84, 97
Application Notes
Application
The quantitative human BNP (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated BNP levels in biological samples. It can be used in studies examining heart failure, cardiovascular disease, and kidney functions. The kit is a valuable tool in cardiology research and the development of new diagnostic methods.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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