Quantitative Human BSEP (Glycosylated) Sandwich ELISA Kit

The quantitative human BSEP (glycosylated) sandwich ELISA kit is designed to detect human bile salt export pump (BSEP) levels. BSEP is a membrane transporter protein primarily expressed in hepatocytes, responsible for the efflux of bile salts from liver cells into bile. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 37.123 pg/mL, which can accurately detect low concentrations of BSEP in the sample.

BSEP

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

37.123 pg/mL

Quantitative sandwich ELISA

100 pg/mL-4000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Bile salt export pump

BSEP; Bile salt export pump; ABCB11; ATP-Binding cassette sub-family B member 11; BRIC2

109, 116, 122, 125

The quantitative human BSEP (glycosylated) sandwich ELISA kit is valuable for research investigating cholestatic liver diseases, drug-induced liver injury, and the mechanisms of bile salt transport. The quantitative data obtained from this kit allows for precise analysis of BSEP levels, contributing to a better understanding of its role in liver function and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.