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Quantitative Human BSEP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX111)

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1×48 T
1×96 T

Product Description The quantitative human BSEP (glycosylated) sandwich ELISA kit is designed to detect human bile salt export pump (BSEP) levels. BSEP is a membrane transporter protein primarily expressed in hepatocytes, responsible for the efflux of bile salts from liver cells into bile. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 37.123 pg/mL, which can accurately detect low concentrations of BSEP in the sample.
Target BSEP
N-Glycosylation Site 109, 116, 122, 125
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 37.123 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 100 pg/mL-4000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Bile salt export pump
Alternate Names BSEP; Bile salt export pump; ABCB11; ATP-Binding cassette sub-family B member 11; BRIC2
Uniprot No. O95342
Application The quantitative human BSEP (glycosylated) sandwich ELISA kit is valuable for research investigating cholestatic liver diseases, drug-induced liver injury, and the mechanisms of bile salt transport. The quantitative data obtained from this kit allows for precise analysis of BSEP levels, contributing to a better understanding of its role in liver function and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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