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Quantitative Human CDC42 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX490

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Specifications
Product Description
The quantitative human CDC42 (glycosylated) sandwich ELISA kit is designed to detect human cell division cycle protein 42 (CDC42) levels. CDC42 is a small GTPase protein that belongs to the Ras superfamily, which plays a crucial role in regulating various cellular processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.101 ng/mL, which can accurately detect low concentrations of CDC42 in the sample.
Target
CDC42
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.101 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cell division cycle protein 42
Alternate Names
CDC42; Cell division cycle protein 42; CDC42Hs; G25K; G25K GTP-binding protein
Uniprot No.
O-Glycosylation Site
32, 35
Application Notes
Application
The quantitative human CDC42 (glycosylated) sandwich ELISA kit is used to measure glycosylated CDC42 levels in samples. This measurement is important in research related to cell signaling. The kit is applied in studies of cellular processes, which helps in understanding the role of CDC42.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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