Quantitative Human CHRNα3 (Glycosylated) Sandwich ELISA Kit

The quantitative human CHRNα3 (glycosylated) sandwich ELISA kit is designed to detect human cholinergic receptor, nicotinic, α3 (CHRNα3) levels. CHRNα3 is a subunit of the nicotinic acetylcholine receptor. This receptor is a ligand-gated ion channel. CHRNα3 plays a role in neurotransmission. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of CHRNα3 in the sample.

CHRNα3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cholinergic receptor, nicotinic, α3

CHRNα3; Cholinergic receptor, nicotinic, α3; CHRN-A3; NAChRA3; Neuronal acetylcholine receptor α3

55, 172

The quantitative human CHRNα3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CHRNα3 levels. It is useful for studying the role of CHRNα3 in synaptic transmission.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.