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Quantitative Human DSPP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX865)

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1×48 T
1×96 T

Product Description The quantitative human DSPP (glycosylated) sandwich ELISA kit is designed to detect human dentin sialophosphoprotein (DSPP) levels. DSPP is a precursor protein that is processed into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). DSPP is predominantly expressed in odontoblasts. It plays a crucial role in dentin formation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.681 ng/mL, which can accurately detect low concentrations of DSPP in the sample.
Target DSPP
N-Glycosylation Site 41, 49, 81, 130, 150, 190, 191, 209, 222, 275, 336, 387
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.681 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.75 ng/mL-85 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Dentin sialophosphoprotein
Alternate Names DSPP; Dentin sialophosphoprotein; DSP; DFNA39; DPP; Dentin phosphoprotein
Uniprot No. Q9NZW4
Application The quantitative human DSPP (glycosylated) sandwich ELISA kit is used to measure DSPP levels in biological samples. This kit is applicable in studies examining dentin formation and tooth development. It is also relevant to studies investigating the role of DSPP in the biomineralization process and its involvement in dental disorders.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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