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Quantitative Human EFNA3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX459

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Specifications
Product Description
The quantitative human EFNA3 (glycosylated) sandwich ELISA kit is designed to detect human ephrin A3 (EFNA3) levels. EFNA3 is a protein that belongs to the ephrin family. Ephrins are cell surface proteins involved in cell signaling. EFNA3 interacts with Eph receptors. This interaction plays a role in various developmental processes. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.104 ng/mL, which can accurately detect low concentrations of EFNA3 in the sample.
Target
EFNA3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.104 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin A3
Alternate Names
EFNA3; Ephrin A3; EPLG3; EHK1 ligand; EPH-related receptor tyrosine kinase ligand 3
Uniprot No.
N-Glycosylation Site
38, 67, 100
Application Notes
Application
The quantitative human EFNA3 (glycosylated) sandwich ELISA kit is used to measure glycosylated EFNA3 levels in samples. This measurement is important in research related to cell signaling. The kit is applied in studies of developmental biology, which helps in understanding the role of EFNA3.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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