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Quantitative Human EFNB2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX461

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Specifications
Product Description
The quantitative human EFNB2 (glycosylated) sandwich ELISA kit is designed to detect human ephrin B2 (EFNB2) levels. EFNB2 is a protein belonging to the transmembrane ephrin family. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.078 ng/mL, which can accurately detect low concentrations of EFNB2 in the sample.
Target
EFNB2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.078 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin B2
Alternate Names
EFNB2; Ephrin B2; EPLG5; LERK5; Eph-related receptor tyrosine kinase ligand 5
Uniprot No.
N-Glycosylation Site
36, 139
Application Notes
Application
The quantitative human EFNB2 (glycosylated) sandwich ELISA kit is used to measure glycosylated EFNB2 levels in samples. This measurement is important in research related to vascular biology. The kit is applied in studies of blood vessel development, which helps in understanding the role of EFNB2.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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