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Quantitative Human EPHA4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX467)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EPHA4 (glycosylated) sandwich ELISA kit is designed to detect human ephrin type A receptor 4 (EPHA4) levels. EPHA4 is another receptor tyrosine kinase that interacts with ephrin-A ligands. Similar to EPHA5, EPHA4 signaling mediates cell-cell communication. This process is crucial for various developmental events. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.122 ng/mL, which can accurately detect low concentrations of EPHA4 in the sample.
Target EPHA4
N-Glycosylation Site 235, 34, 408, 545
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.122 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ephrin type A receptor 4
Alternate Names EPHA4; Ephrin type A receptor 4; HEK8; SEK; EPH-like kinase 8; Tyrosine-protein kinase receptor SEK
Uniprot No. P54764
Application The quantitative human EPHA4 (glycosylated) sandwich ELISA kit is used to measure glycosylated EPHA4 levels in samples. This measurement is important in research related to cell signaling. The kit is applied in studies of developmental biology, which helps in understanding the role of EPHA4.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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