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Quantitative Human EPHA4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX467

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Specifications
Product Description
The quantitative human EPHA4 (glycosylated) sandwich ELISA kit is designed to detect human ephrin type A receptor 4 (EPHA4) levels. EPHA4 is another receptor tyrosine kinase that interacts with ephrin-A ligands. Similar to EPHA5, EPHA4 signaling mediates cell-cell communication. This process is crucial for various developmental events. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.122 ng/mL, which can accurately detect low concentrations of EPHA4 in the sample.
Target
EPHA4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.122 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Ephrin type A receptor 4
Alternate Names
EPHA4; Ephrin type A receptor 4; HEK8; SEK; EPH-like kinase 8; Tyrosine-protein kinase receptor SEK
Uniprot No.
N-Glycosylation Site
235, 34, 408, 545
Application Notes
Application
The quantitative human EPHA4 (glycosylated) sandwich ELISA kit is used to measure glycosylated EPHA4 levels in samples. This measurement is important in research related to cell signaling. The kit is applied in studies of developmental biology, which helps in understanding the role of EPHA4.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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