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Quantitative Human EPHA5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX466)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human EPHA5 (glycosylated) sandwich ELISA kit is designed to detect human ephrin type A receptor 5 (EPHA5) levels. EPHA5 is a receptor tyrosine kinase that binds ephrin-A ligands. EphA5 signaling is involved in cell-cell communication. This interaction plays a role in various developmental processes, including axon guidance. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of EPHA5 in the sample.
Target EPHA5
N-Glycosylation Site 264, 299, 369, 423, 436, 461
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.120 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Ephrin type A receptor 5
Alternate Names EPHA5; Ephrin type A receptor 5; TYRO4; EHK1; EPH homology kinase 1
Uniprot No. P54756
Application The quantitative human EPHA5 (glycosylated) sandwich ELISA kit is designed to measure glycosylated EPHA5 levels in samples. This measurement is valuable in research related to cell signaling. The kit is applied in studies of neural development, which helps in understanding the role of EPHA5.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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