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Quantitative Human FGFR1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX230

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Specifications
Product Description
The quantitative human FGFR1 (glycosylated) sandwich ELISA kit is designed to detect human fibroblast growth factor receptor 1 (FGFR1) levels. FGFR1 is a tyrosine kinase receptor involved in cell proliferation, differentiation, and angiogenesis, playing a crucial role in development and tissue repair. Its dysregulation is implicated in various cancers and skeletal disorders. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.090 ng/mL, which can accurately detect low concentrations of FGFR1 in the sample.
Target
FGFR1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.090 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Fibroblast growth factor receptor 1
Alternate Names
FGFR1; Fibroblast growth factor receptor 1; CD331; FLT2, KAL2; Fms-Related tyrosine kinase-2
Uniprot No.
N-Glycosylation Site
77, 117, 227, 240, 264, 296, 317, 330
Application Notes
Application
The quantitative human FGFR1 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer biology, skeletal development, and wound healing. Researchers utilize this ELISA to quantify FGFR1 levels, providing insights into its role in these processes and potential therapeutic targeting.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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