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Quantitative Human GDF5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX419

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Specifications
Product Description
The quantitative human GDF5 (glycosylated) sandwich ELISA kit is designed to detect human growth differentiation factor 5 (GDF5) levels. GDF5 is a protein belonging to the transforming growth factor-beta (TGF-beta) superfamily. It plays a crucial role in the development of cartilage and joints. GDF5 is involved in regulating cell growth and differentiation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 33 pg/mL, which can accurately detect low concentrations of GDF5 in the sample.
Target
GDF5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
33 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Growth differentiation factor 5
Alternate Names
GDF5; Growth differentiation factor 5; CDMP1; BMP14; Bone morphogenetic protein 14
Uniprot No.
N-Glycosylation Site
189
Application Notes
Application
The quantitative human GDF5 (glycosylated) sandwich ELISA kit is used to measure glycosylated GDF5 levels in samples. This measurement is important in research related to skeletal development and joint disorders. The kit is applied in studies of conditions such as osteoarthritis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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