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Quantitative Human GLUT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX226)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GLUT1 (glycosylated) sandwich ELISA kit is designed to detect human glucose transporter 1 (GLUT1) levels. GLUT1 is a membrane protein that facilitates the transport of glucose across cell membranes, playing a crucial role in cellular glucose uptake. It is widely expressed in various tissues and is essential for maintaining glucose homeostasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.121 ng/mL, which can accurately detect low concentrations of GLUT1 in the sample.
Target GLUT1
N-Glycosylation Site 45
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.121 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.25 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Glucose transporter 1
Alternate Names GLUT1; Glucose transporter 1; SLC2A1; GLUT; Solute carrier family 2 member 1, facilitated glucose transporter
Uniprot No. P11166
Application The quantitative human GLUT1 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer metabolism, glucose transport disorders, and neurological diseases. Researchers utilize this ELISA to quantify GLUT1 levels, providing insights into glucose uptake regulation and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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