Quantitative Human GRIA4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX431)

Product Description	The quantitative human GRIA4 (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, AMPA4 (GRIA4) levels. GRIA4 is a subunit of the AMPA receptor. AMPA receptors are ligand-gated ion channels that mediate fast synaptic transmission in the brain. GRIA4 contributes to the formation of functionsal AMPA receptors. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.152 ng/mL, which can accurately detect low concentrations of GRIA4 in the sample.
Target	GRIA4
N-Glycosylation Site	52, 56, 258, 371, 407, 414
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.152 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Glutamate receptor, ionotropic, AMPA4
Alternate Names	GRIA4; Glutamate receptor, ionotropic, AMPA4; GLUR4; GLURD; AMPA-selective glutamate receptor 4
Uniprot No.	P48058

Application	The quantitative human GRIA4 (glycosylated) sandwich ELISA kit is used to measure glycosylated GRIA4 levels in samples. This measurement is important in neuroscience research, particularly for studying synaptic functions and neurological disorders. The kit is applied in investigations of conditions affecting glutamatergic transmission, which helps in understanding the role of GRIA4.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-95%; Plasma sample: n=4, 85-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.