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Quantitative Human GRIA4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX431

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Specifications
Product Description
The quantitative human GRIA4 (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, AMPA4 (GRIA4) levels. GRIA4 is a subunit of the AMPA receptor. AMPA receptors are ligand-gated ion channels that mediate fast synaptic transmission in the brain. GRIA4 contributes to the formation of functionsal AMPA receptors. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.152 ng/mL, which can accurately detect low concentrations of GRIA4 in the sample.
Target
GRIA4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.152 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, AMPA4
Alternate Names
GRIA4; Glutamate receptor, ionotropic, AMPA4; GLUR4; GLURD; AMPA-selective glutamate receptor 4
Uniprot No.
N-Glycosylation Site
52, 56, 258, 371, 407, 414
Application Notes
Application
The quantitative human GRIA4 (glycosylated) sandwich ELISA kit is used to measure glycosylated GRIA4 levels in samples. This measurement is important in neuroscience research, particularly for studying synaptic functions and neurological disorders. The kit is applied in investigations of conditions affecting glutamatergic transmission, which helps in understanding the role of GRIA4.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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