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Quantitative Human GRIN2B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX559

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Specifications
Product Description
The quantitative human GRIN2B (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, N-methyl-D-aspartate 2B (GRIN2B) levels. GRIN2B is a subunit of the NMDA receptor. NMDA receptors are ligand-gated ion channels in nerve cells, crucial for synaptic transmission, learning, and memory. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of GRIN2B in the sample.
Target
GRIN2B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, N-methyl-D-aspartate 2B
Alternate Names
GRIN2B; Glutamate receptor, ionotropic, N-methyl-D-aspartate 2B; NMDAR2B; NR2B; N-Methyl D-aspartate receptor subtype 2B
Uniprot No.
N-Glycosylation Site
74, 341, 348, 444, 491, 542, 688
Application Notes
Application
The quantitative human GRIN2B (glycosylated) sandwich ELISA kit is valuable for neuroscience and neuropharmacology research, especially in studying glutamate signaling and synaptic plasticity. Researchers can measure GRIN2B levels in brain tissue. Applications include studies of neurological disorders, brain development, and cognitive functions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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