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Quantitative Human GRIN2D (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX43

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Specifications
Product Description
The quantitative human GRIN2D (glycosylated) sandwich ELISA kit is designed to detect human glutamate receptor, ionotropic, N-methyl-D-aspartate 2D (GRIN2D) levels. GRIN2D is a subunit of the NMDA receptor, a glutamate receptor crucial for synaptic plasticity, neuronal development, and various cognitive functions. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.274 ng/mL, which can accurately detect low concentrations of GRIN2D in the sample.
Target
GRIN2D
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.274 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.5 ng/mL-20 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Glutamate receptor, ionotropic, N-methyl-D-aspartate 2D
Alternate Names
GRIN2D; Glutamate receptor, ionotropic, N-methyl-D-aspartate 2D; EB11; NMDAR2D; NR2D
Uniprot No.
N-Glycosylation Site
92, 352, 366, 384, 467, 569
Application Notes
Application
The quantitative human GRIN2D (glycosylated) sandwich ELISA kit is useful for quantifying GRIN2D expression, aiding in research that explores the involvement of GRIN2D in conditions such as epilepsy, intellectual disability, and schizophrenia. Researchers utilize this tool to deepen their understanding of GRIN2D's function within the nervous system.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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