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Quantitative Human HJV (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX657

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Specifications
Product Description
The quantitative human HJV (glycosylated) sandwich ELISA kit is designed to detect human hemojuvelin (HJV) levels. HJV is a protein that plays a crucial role in regulating iron homeostasis. It functions as a co-receptor for bone morphogenetic proteins (BMPs) in the liver. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 14.891 pg/mL, which can accurately detect low concentrations of HJV in the sample.
Target
HJV
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
14.891 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Hemojuvelin
Alternate Names
HJV; Hemojuvelin; HFE2A; HJV; RGMC; Haemojuvelin; RGM domain family member C
Uniprot No.
N-Glycosylation Site
118, 213, 372
Application Notes
Application
The quantitative human HJV (glycosylated) sandwich ELISA kit is used to determine the HJV levels in biological samples. The kit is applicable in studies of iron metabolism, hepcidin regulation, and hemochromatosis. It is useful in fields such as hematology and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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