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Quantitative Human IgA2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX144

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Specifications
Product Description
The quantitative human IgA2 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin A2 (IgA2) levels. IgA2 is a subclass of IgA, primarily found in mucosal secretions, playing a crucial role in mucosal immunity. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.892 µg/mL, which can accurately detect low concentrations of IgA2 in the sample.
Target
IgA2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.892 µg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
3 µg/mL-60 µg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Immunoglobulin A2
Alternate Names
IgA2; Immunoglobulin A2; IGHA2; Immunoglobulin heavy constant α2
Uniprot No.
N-Glycosylation Site
47, 92, 131, 205, 327
Application Notes
Application
The quantitative human IgA2 (glycosylated) sandwich ELISA kit is valuable in research investigating mucosal immunity, inflammatory bowel disease, and other conditions involving mucosal defense. Researchers utilize this ELISA to quantify IgA2 levels, providing insights into its specific role in mucosal protection and related pathologies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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