Quantitative Human KERA (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX81)

Name: Quantitative Human KERA (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX81
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human KERA (glycosylated) sandwich ELISA kit is designed to detect human keratocan (KERA) levels. KERA is a small leucine-rich proteoglycan found primarily in the cornea, playing a crucial role in maintaining corneal transparency and structure. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of KERA in the sample.
Target	KERA
N-Glycosylation Site	93, 167, 222, 298
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.140 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-15 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Keratocan
Alternate Names	KERA; Keratocan; CNA2; SLRR2B; Keratocan proteoglycan
Uniprot No.	O60938

Application	The quantitative human KERA (glycosylated) sandwich ELISA kit is valuable for research investigating corneal diseases, such as keratoconus and corneal dystrophies. The quantitative data obtained from this kit allows for precise analysis of KERA levels, aiding in understanding its role in corneal health and disease pathogenesis.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 85-100%; Plasma sample: n=4, 80-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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