Quantitative Human KERA (Glycosylated) Sandwich ELISA Kit

The quantitative human KERA (glycosylated) sandwich ELISA kit is designed to detect human keratocan (KERA) levels. KERA is a small leucine-rich proteoglycan found primarily in the cornea, playing a crucial role in maintaining corneal transparency and structure. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.140 ng/mL, which can accurately detect low concentrations of KERA in the sample.

KERA

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.140 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Keratocan

KERA; Keratocan; CNA2; SLRR2B; Keratocan proteoglycan

93, 167, 222, 298

The quantitative human KERA (glycosylated) sandwich ELISA kit is valuable for research investigating corneal diseases, such as keratoconus and corneal dystrophies. The quantitative data obtained from this kit allows for precise analysis of KERA levels, aiding in understanding its role in corneal health and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.