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Quantitative Human KL (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX663

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human KL (glycosylated) sandwich ELISA kit is designed to detect human klotho (KL) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.046 ng/mL, which can accurately detect low concentrations of KL in the sample.
Target
KL
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated klotho antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.046 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.16 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Klotho
Alternate Names
Klotho; Klotho peptide; EC 3.2.1.31; KL
Uniprot No.
Q9UEF7
N-Glycosylation Site
106, 159, 283, 344, 607, 612, 694
Application Notes
Application
The quantitative human KL (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of KL, helping to understand its role in developmental biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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