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Quantitative Human KL (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX663)

Datasheet
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1×96 T
5×96 T
10×96 T

Product Description The quantitative human KL (glycosylated) sandwich ELISA kit is designed to detect human klotho (KL) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.046 ng/mL, which can accurately detect low concentrations of KL in the sample.
Target KL
N-Glycosylation Site 106, 159, 283, 344, 607, 612, 694
Sample Types Tissue homogenates, serum, plasma
Sample Volume 50 μL-100 μL
Sensitivity 0.046 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.16 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Klotho
Alternate Names Klotho; Klotho peptide; EC 3.2.1.31; KL
Uniprot No. Q9UEF7
Application The quantitative human KL (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of KL, helping to understand its role in developmental biology.
Kit Components Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated klotho antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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