Quantitative Human KRT18 (Glycosylated) Sandwich ELISA Kit

The quantitative human KRT18 (glycosylated) sandwich ELISA kit is designed to detect human keratin 18 (KRT18) levels. KRT18 is an intermediate filament protein expressed in simple epithelial tissues, playing a crucial role in maintaining cellular structure and integrity. It is often used as a biomarker for epithelial cell death and tissue damage. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.089 ng/mL, which can accurately detect low concentrations of KRT18 in the sample.

KRT18

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.089 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Keratin 18

KRT18; Keratin 18; CYK18; Cytokeratin 18

30, 31, 49

The quantitative human KRT18 (glycosylated) sandwich ELISA kit is valuable in research investigating liver diseases, cancer, and other conditions involving epithelial cell damage. Researchers utilize this ELISA to quantify KRT18 levels, providing insights into tissue injury, disease progression, and potential therapeutic responses.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.