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Quantitative Human LASS6 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX655

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Specifications
Product Description
The quantitative human LASS6 (glycosylated) sandwich ELISA kit is designed to detect human longevity assurance homolog 6 (LASS6) levels. LASS6 is an enzyme that catalyzes the synthesis of ceramide, a bioactive lipid. Specifically, LASS6 is involved in the formation of very long-chain ceramides. These ceramides play a role in various cellular processes, including cell growth, differentiation, and apoptosis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of LASS6 in the sample.
Target
LASS6
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.099 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Longevity assurance homolog 6
Alternate Names
LASS6; Longevity assurance homolog 6; CerS6; Ceramide synthase 6
Uniprot No.
N-Glycosylation Site
18
Application Notes
Application
The quantitative human LASS6 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LASS6 levels of this enzyme in biological samples. The kit is applicable in studies of lipid metabolism, ceramide synthesis, and cell signaling. It is useful in fields such as biochemistry and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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