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Quantitative Human LNPEP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX882

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Specifications
Product Description
The quantitative human LNPEP (glycosylated) sandwich ELISA kit is designed to detect human leucyl aminopeptidase (LNPEP) levels. LNPEP is an aminopeptidase that cleaves amino acids from the N-terminus of proteins. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.084 ng/mL, which can accurately detect low concentrations of LNPEP in the sample.
Target
LNPEP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.084 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Leucyl aminopeptidase
Alternate Names
LNPEP; Leucyl aminopeptidase; PLAP; OTASE; Oxytocinase; Placental leucine aminopeptidase
Uniprot No.
N-Glycosylation Site
145, 184, 215, 256, 266, 368, 374, 448, 525, 578, 598, 664, 682, 760, 834, 850, 989
Application Notes
Application
The quantitative human LNPEP (glycosylated) sandwich ELISA kit is used to measure LNPEP levels in biological samples. This kit is useful in studies examining peptide metabolism and hormone processing. It is also relevant to studies investigating the role of LNPEP in regulating the levels of oxytocin and vasopressin and its potential involvement in various physiological functions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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