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Quantitative Human MAPt (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX219)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MAPt (glycosylated) sandwich ELISA kit is designed to detect human microtubule associated protein tau (MAPt) levels. MAPt is a protein that stabilizes microtubules in neurons, playing a crucial role in axonal transport and neuronal function. Its abnormal aggregation is a hallmark of several neurodegenerative diseases, including Alzheimer's disease. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.096 ng/mL, which can accurately detect low concentrations of MAPt in the sample.
Target MAPt
N-Glycosylation Site 87, 383, 467, 480, 491, 542, 551, 576, 597, 598, 664, 670, 686
O-Glycosylation Site 525, 555, 717
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.096 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Microtubule associated protein tau
Alternate Names MAPt; Microtubule associated protein Tau; DDPAC; MSTD; Neurofibrillary tangle protein; Tau protein
Uniprot No. P10636
Application The quantitative human MAPt (glycosylated) sandwich ELISA kit is valuable in research investigating neurodegenerative diseases, particularly Alzheimer's disease and other tauopathies. Researchers utilize this ELISA to quantify MAPt levels, providing insights into disease progression and potential diagnostic or prognostic markers.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-110%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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