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Quantitative Human MEGF8 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX668

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Specifications
Product Description
The quantitative human MEGF8 (glycosylated) sandwich ELISA kit is designed to detect human multiple epidermal growth factor-like domains protein 8 (MEGF8) levels. MEGF8 is a transmembrane protein that contains multiple epidermal growth factor (EGF)-like domains. Proteins with EGF-like domains are often involved in cell-cell interactions and signaling pathways. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.094 ng/mL, which can accurately detect low concentrations of MEGF8 in the sample.
Target
MEGF8
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
0.094 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Multiple epidermal growth factor-like domains protein 8
Alternate Names
MEGF8; Multiple epidermal growth factor-like domains protein 8; CRPT2; EGFL4
Uniprot No.
N-Glycosylation Site
50, 217, 1048, 1271, 2066, 2229
Application Notes
Application
The quantitative human MEGF8 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated MEGF8 levels in biological samples. The kit is applicable in studies of neuroscience, neuronal development, and synapse formation. It is useful in fields such as neurobiology and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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