Quantitative Human MEP1α (Glycosylated) Competitive ELISA Kit

The quantitative human MEP1α (glycosylated) competitive ELISA kit is designed to detect human meprin 1α (MEP1α) levels. MEP1α is a zinc metalloproteinase enzyme, which belongs to the astacin family of proteases. MEP1α is expressed on the cell surface of various tissues, including the kidney and intestine. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 19.274 pg/mL, which can accurately detect low concentrations of MEP1α in the sample.

MEP1α

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

19.274 pg/mL

Quantitative competitive ELISA

65 pg/mL-3500 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Meprin 1α

MEP1α; Meprin 1α; PPHA; Endopeptidase-2; PABA peptide hydrolase

140, 222, 258, 414, 440, 447, 539

630

The quantitative human MEP1α (glycosylated) competitive ELISA kit is designed for the quantitative measurement of glycosylated MEP1α levels in biological samples. The kit is applicable in studies of extracellular matrix biology, tissue remodeling, and inflammation. It is useful in fields such as cell biology and oncology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.