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Quantitative Human MEP1α (Glycosylated) Competitive ELISA Kit (CAT#: GLJF-0525-HX618)

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1×48 T
1×96 T

Product Description The quantitative human MEP1α (glycosylated) competitive ELISA kit is designed to detect human meprin 1α (MEP1α) levels. MEP1α is a zinc metalloproteinase enzyme, which belongs to the astacin family of proteases. MEP1α is expressed on the cell surface of various tissues, including the kidney and intestine. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 19.274 pg/mL, which can accurately detect low concentrations of MEP1α in the sample.
Target MEP1α
N-Glycosylation Site 140, 222, 258, 414, 440, 447, 539
O-Glycosylation Site 630
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 19.274 pg/mL
Detection Principle Quantitative competitive ELISA
Detection Range 65 pg/mL-3500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Meprin 1α
Alternate Names MEP1α; Meprin 1α; PPHA; Endopeptidase-2; PABA peptide hydrolase
Uniprot No. Q16819
Application The quantitative human MEP1α (glycosylated) competitive ELISA kit is designed for the quantitative measurement of glycosylated MEP1α levels in biological samples. The kit is applicable in studies of extracellular matrix biology, tissue remodeling, and inflammation. It is useful in fields such as cell biology and oncology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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