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Quantitative Human MEP1β (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX619)

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1×48 T
1×96 T

Product Description The quantitative human MEP1β (glycosylated) sandwich ELISA kit is designed to detect human meprin 1β (MEP1β) levels. MEP1β is another subunit of the meprin A metalloproteinase complex. Similar to MEP1α, it is a zinc-dependent protease. MEP1β can form heterodimers with MEP1α, influencing the enzymatic activity and substrate specificity of the complex. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of MEP1β in the sample.
Target MEP1β
N-Glycosylation Site 218, 254, 370, 421, 436, 445, 547, 592
O-Glycosylation Site 593, 594, 599, 603
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Meprin 1β
Alternate Names MEP1β; Meprin 1β; PPH Beta; Meprin B
Uniprot No. Q16820
Application The quantitative human MEP1β (glycosylated) sandwich ELISA kit is used to quantitatively measures glycosylated MEP1β levels in biological samples. It proves valuable in research focused on extracellular matrix dynamics, tissue repair, and proteolytic enzyme activity. This kit finds application in fields such as cell biology, biochemistry, and pathological studies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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