Quantitative Human MEP1β (Glycosylated) Sandwich ELISA Kit

The quantitative human MEP1β (glycosylated) sandwich ELISA kit is designed to detect human meprin 1β (MEP1β) levels. MEP1β is another subunit of the meprin A metalloproteinase complex. Similar to MEP1α, it is a zinc-dependent protease. MEP1β can form heterodimers with MEP1α, influencing the enzymatic activity and substrate specificity of the complex. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of MEP1β in the sample.

MEP1β

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Meprin 1β

MEP1β; Meprin 1β; PPH Beta; Meprin B

218, 254, 370, 421, 436, 445, 547, 592

593, 594, 599, 603

The quantitative human MEP1β (glycosylated) sandwich ELISA kit is used to quantitatively measures glycosylated MEP1β levels in biological samples. It proves valuable in research focused on extracellular matrix dynamics, tissue repair, and proteolytic enzyme activity. This kit finds application in fields such as cell biology, biochemistry, and pathological studies.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.