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Quantitative Human PDCD1LG2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX775

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Specifications
Product Description
The quantitative human PDCD1LG2 (glycosylated) sandwich ELISA kit is designed to detect human programmed cell death protein 1 ligand 2 (PDCD1LG2) levels. PDCD1LG2 is a protein that interacts with PD-1. PD-1 is an immune checkpoint protein. PDCD1LG2 plays a role in regulating immune responses, which helps to prevent excessive immune activation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.082 ng/mL, which can accurately detect low concentrations of PDCD1LG2 in the sample.
Target
PDCD1LG2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.082 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Programmed cell death protein 1 ligand 2
Alternate Names
PDCD1LG2; Programmed cell death protein 1 ligand 2; CD273; PDCD1-L2; PDL2
Uniprot No.
N-Glycosylation Site
37, 64, 157, 163, 189
Application Notes
Application
The quantitative human PDCD1LG2 (glycosylated) sandwich ELISA kit is developed for the quantitative analysis of glycosylated PDCD1LG2 in samples. This measurement is valuable for research on immune regulation and immune checkpoints. The kit also has potential applications in investigations of cancer immunotherapy.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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