Quantitative Human PFKM (Glycosylated) Sandwich ELISA Kit

The quantitative human PFKM (glycosylated) sandwich ELISA kit is designed to detect human phosphofructokinase (PFKM) levels. PFKM is a key enzyme in glycolysis, catalyzing the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a crucial step in glucose metabolism. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PFKM in the sample.

PFKM

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates

100 μL

0.120 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Phosphofructokinase

PFKM; Phosphofructokinase; PFK1; Phosphofructokinase 1; Fructose-6-phosphate 1-phosphotransferase

530

The quantitative human PFKM (glycosylated) sandwich ELISA kit is valuable in research investigating glucose metabolism, cancer biology, and muscle diseases, where PFKM activity and expression are relevant. Researchers utilize this ELISA to quantify PFKM levels, providing insights into its role in cellular energy production and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.