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Quantitative Human PFKM (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX199)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PFKM (glycosylated) sandwich ELISA kit is designed to detect human phosphofructokinase (PFKM) levels. PFKM is a key enzyme in glycolysis, catalyzing the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a crucial step in glucose metabolism. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PFKM in the sample.
Target PFKM
O-Glycosylation Site 530
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.120 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Phosphofructokinase
Alternate Names PFKM; Phosphofructokinase; PFK1; Phosphofructokinase 1; Fructose-6-phosphate 1-phosphotransferase
Uniprot No. P08237
Application The quantitative human PFKM (glycosylated) sandwich ELISA kit is valuable in research investigating glucose metabolism, cancer biology, and muscle diseases, where PFKM activity and expression are relevant. Researchers utilize this ELISA to quantify PFKM levels, providing insights into its role in cellular energy production and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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