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Quantitative Human PPAR-γ (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX397

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Specifications
Product Description
The quantitative human PPAR-γ (glycosylated) sandwich ELISA kit is designed to detect human peroxisome proliferator activated receptor γ (PPAR-γ) levels. PPAR-γ is a nuclear receptor protein, which regulates the expression of genes involved in adipocyte differentiation. PPAR-γ plays a role in regulating glucose metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.078 ng/mL, which can accurately detect low concentrations of PPAR-γ in the sample.
Target
PPAR-γ
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.078 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Peroxisome proliferator activated receptor γ
Alternate Names
PPAR-γ; Peroxisome proliferator activated receptor γ; PPAR-G; NR1C3; Nuclear receptor subfamily 1 group C member 3; Peroxisome proliferator activated receptor gamma
Uniprot No.
O-Glycosylation Site
84
Application Notes
Application
The quantitative human PPAR-γ (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PPAR-γ levels. It is useful for studying the role of PPAR-γ in insulin sensitivity.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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