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Quantitative Human PPAR-γ (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX397)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PPAR-γ (glycosylated) sandwich ELISA kit is designed to detect human peroxisome proliferator activated receptor γ (PPAR-γ) levels. PPAR-γ is a nuclear receptor protein, which regulates the expression of genes involved in adipocyte differentiation. PPAR-γ plays a role in regulating glucose metabolism. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.078 ng/mL, which can accurately detect low concentrations of PPAR-γ in the sample.
Target PPAR-γ
O-Glycosylation Site 84
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.078 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Peroxisome proliferator activated receptor γ
Alternate Names PPAR-γ; Peroxisome proliferator activated receptor γ; PPAR-G; NR1C3; Nuclear receptor subfamily 1 group C member 3; Peroxisome proliferator activated receptor gamma
Uniprot No. P37231
Application The quantitative human PPAR-γ (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PPAR-γ levels. It is useful for studying the role of PPAR-γ in insulin sensitivity.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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