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Quantitative Human PRCP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX415

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Specifications
Product Description
The quantitative human PRCP (glycosylated) sandwich ELISA kit is designed to detect human prolylcarboxypeptidase (PRCP) levels. PRCP is an enzyme that cleaves proline residues from the C-terminus of peptides. It is involved in the degradation of certain bioactive peptides, including angiotensin II. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma, urine. Its sensitivity is 32.846 pg/mL, which can accurately detect low concentrations of PRCP in the sample.
Target
PRCP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma, urine
Sample Volume
100 μL
Sensitivity
32.846 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Prolylcarboxypeptidase
Alternate Names
PRCP; Prolylcarboxypeptidase; PCP; Angiotensinase C; Lysosomal carboxypeptidase C
Uniprot No.
N-Glycosylation Site
47, 101, 317, 336, 345, 415
Application Notes
Application
The quantitative human PRCP (glycosylated) sandwich ELISA kit is used to measure glycosylated PRCP levels in samples. This measurement is important in research related to cardiovascular functions and inflammation. The kit is applied in studies of conditions such as hypertension and inflammatory diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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