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Quantitative Human RHO (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX194

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Specifications
Product Description
The quantitative human RHO (glycosylated) sandwich ELISA kit is designed to detect human rhodopsin (RHO) levels. RHO is a light-sensitive G protein-coupled receptor located in the rod cells of the retina, essential for vision in low-light conditions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.766 ng/mL, which can accurately detect low concentrations of RHO in the sample.
Target
RHO
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.766 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.5 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Rhodopsin
Alternate Names
RHO; Rhodopsin; OPN2; RP4; Opsin 2; Retinitis pigmentosa 4
Uniprot No.
N-Glycosylation Site
2, 15
Application Notes
Application
The quantitative human RHO (glycosylated) sandwich ELISA kit is valuable in research investigating retinal diseases such as retinitis pigmentosa, night blindness, and other vision-related disorders. Researchers utilize this ELISA to quantify RHO levels, providing insights into its expression and potential diagnostic or therapeutic applications related to visual function.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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