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Quantitative Human sCD14 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX98

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human sCD14 (glycosylated) sandwich ELISA kit is designed to detect human soluble cluster of differentiation 14 (sCD14) levels. It is suitable for various biological samples such as cell culture supernates, serum, plasma, or urine. Its sensitivity is 0.231 ng/mL, which can accurately detect low concentrations of sCD14 in the sample.
Target
CD14
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated CD14 antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Cell culture supernates, serum, plasma, urine
Sample Volume
50 μL-100 μL
Sensitivity
0.231 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.25 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Soluble cluster of differentiation 14
Alternate Names
Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14; Soluble cluster of differentiation 14; CD14 antigen; CD14 molecule; CD14
Uniprot No.
P08571
N-Glycosylation Site
37, 151, 282, 323
O-Glycosylation Site
336
Application Notes
Application
The quantitative human sCD14 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of sCD14, helping to understand its role in immunology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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