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Quantitative Human SEMA3F (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX561)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3F (SEMA3F) levels. SEMA3F is a secreted protein in the semaphorin family, which acts as a chemorepellent, guiding axons away from certain regions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 6.194 pg/mL, which can accurately detect low concentrations of SEMA3F in the sample.
Target SEMA3F
N-Glycosylation Site 53, 126
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 6.194 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 20 pg/mL-800 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Semaphorin 3F
Alternate Names SEMA3F; Semaphorin 3F; SEMA-IV; SEMA4
Uniprot No. Q13275
Application The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is a valuable tool for research in cell and developmental biology, especially in studies of axon guidance and angiogenesis. Researchers can measure SEMA3F levels in tissues and cell culture. Applications include studies of neurological development, cancer, and tissue morphogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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