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| Size | Qty | Add To Basket |
|---|---|---|
| 1×48 T | ||
| 1×96 T |
| Product Description | The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3F (SEMA3F) levels. SEMA3F is a secreted protein in the semaphorin family, which acts as a chemorepellent, guiding axons away from certain regions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 6.194 pg/mL, which can accurately detect low concentrations of SEMA3F in the sample. |
| Target | SEMA3F |
| N-Glycosylation Site | 53, 126 |
| Sample Types | Tissue homogenates, serum, plasma |
| Sample Volume | 100 μL |
| Sensitivity | 6.194 pg/mL |
| Detection Principle | Quantitative sandwich ELISA |
| Detection Range | 20 pg/mL-800 pg/mL |
| Detection Time | 1 h-5 h |
| Detection Wavelength | 450 nm |
| Storage | Store at 2-8°C for long term storage. |
| Species | Human |
| Full Name | Semaphorin 3F |
| Alternate Names | SEMA3F; Semaphorin 3F; SEMA-IV; SEMA4 |
| Uniprot No. | Q13275 |
| Application | The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is a valuable tool for research in cell and developmental biology, especially in studies of axon guidance and angiogenesis. Researchers can measure SEMA3F levels in tissues and cell culture. Applications include studies of neurological development, cancer, and tissue morphogenesis. |
| Kit Components | Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution |
| Precision | Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%; |
| Recovery | Serum sample: n=5, 80-100%; Plasma sample: n=4, 80-95%; |
| Standard Curve | ![]() The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested. |