Quantitative Human SEMA3F (Glycosylated) Sandwich ELISA Kit

The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3F (SEMA3F) levels. SEMA3F is a secreted protein in the semaphorin family, which acts as a chemorepellent, guiding axons away from certain regions. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 6.194 pg/mL, which can accurately detect low concentrations of SEMA3F in the sample.

SEMA3F

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

6.194 pg/mL

Quantitative sandwich ELISA

20 pg/mL-800 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Semaphorin 3F

SEMA3F; Semaphorin 3F; SEMA-IV; SEMA4

53, 126

The quantitative human SEMA3F (glycosylated) sandwich ELISA kit is a valuable tool for research in cell and developmental biology, especially in studies of axon guidance and angiogenesis. Researchers can measure SEMA3F levels in tissues and cell culture. Applications include studies of neurological development, cancer, and tissue morphogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.