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Quantitative Human UPK3A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX94

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Specifications
Product Description
The quantitative human UPK3A (glycosylated) sandwich ELISA kit is designed to detect human uroplakin 3A (UPK3A) levels. UPK3A is a major component of the asymmetric unit membrane (AUM) of urothelial cells, playing a critical role in the barrier function of the bladder epithelium. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.139 ng/mL, which can accurately detect low concentrations of UPK3A in the sample.
Target
UPK3A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.139 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Uroplakin 3A
Alternate Names
UPK3A; Uroplakin 3A; UPIII; UPK3
Uniprot No.
N-Glycosylation Site
74, 139, 170
Application Notes
Application
The quantitative human UPK3A (glycosylated) sandwich ELISA kit is valuable for research and clinical diagnostics related to bladder cancer, urinary tract infections, and other urothelial disorders. The quantitative data obtained from this kit allows for precise analysis of UPK3A levels, contributing to a better understanding of its role in bladder health and disease pathogenesis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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