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Quantitative Human UPK3A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX94)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human UPK3A (glycosylated) sandwich ELISA kit is designed to detect human uroplakin 3A (UPK3A) levels. UPK3A is a major component of the asymmetric unit membrane (AUM) of urothelial cells, playing a critical role in the barrier function of the bladder epithelium. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.139 ng/mL, which can accurately detect low concentrations of UPK3A in the sample.
Target UPK3A
N-Glycosylation Site 74, 139, 170
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.139 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Uroplakin 3A
Alternate Names UPK3A; Uroplakin 3A; UPIII; UPK3
Uniprot No. O75631
Application The quantitative human UPK3A (glycosylated) sandwich ELISA kit is valuable for research and clinical diagnostics related to bladder cancer, urinary tract infections, and other urothelial disorders. The quantitative data obtained from this kit allows for precise analysis of UPK3A levels, contributing to a better understanding of its role in bladder health and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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