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Quantitative Human WNT10B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX21

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Specifications
Product Description
The quantitative human WNT10B (glycosylated) sandwich ELISA kit is designed to detect human wingless type MMTV integration site family, member 10B (WNT10B) levels. WNT10B is a secreted glycoprotein that plays a critical role in various developmental processes, including adipogenesis, bone formation, and tissue regeneration, by activating Wnt signaling pathways. It is involved in cell proliferation, differentiation, and tissue homeostasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 29.873 pg/mL, which can accurately detect low concentrations of WNT10B in the sample.
Target
WNT10B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
29.873 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
65 pg/mL-3000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless type MMTV integration site family, member 10B
Alternate Names
WNT10B; Wingless type MMTV integration site family, member 10B; WNT-12
Uniprot No.
N-Glycosylation Site
93, 335
Application Notes
Application
The quantitative human WNT10B (glycosylated) sandwich ELISA kit is valuable for researchers investigating metabolic disorders, skeletal diseases, and cancer, where WNT10B signaling is often dysregulated. By quantifying WNT10B levels, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to Wnt pathway modulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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