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Quantitative Mouse TGFB1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1084

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative mouse TGFB1 (glycosylated) sandwich ELISA kit is designed to detect mouse transforming growth factor β1 (TGFB1) levels. It is suitable for various biological samples such as cell culture supernates, serum, or plasma. Its sensitivity is 0.224 ng/mL, which can accurately detect low concentrations of TGFB1 in the sample.
Target
TGFB1
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated TGF-β1 antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.224 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.80 ng/mL-50 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Transforming growth factor β1
Alternate Names
Transforming growth factor β1; Transforming growth factor β-1 proprotein; TGF-β-1; TGFB1
Uniprot No.
P04202
N-Glycosylation Site
82, 136, 176
Application Notes
Application
The quantitative mouse TGFB1 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of TGFB1, helping to understand its role in signal transduction.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 90-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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