Quantitative Rat TNF (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX1127)

Product Description	The quantitative rat TNF (glycosylated) sandwich ELISA kit is designed to detect rat tumor necrosis factor (TNF) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 1.625 pg/mL, which can accurately detect low concentrations of TNF in the sample.
Target	TNF
N-Glycosylation Site	86
O-Glycosylation Site	83
Sample Types	Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	1.625 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	6.50 pg/mL-400 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Rat
Full Name	Tumor necrosis factor
Alternate Names	Tumor necrosis factor; TNF; TNFA; TNFSF2; TNF-α; Tumor necrosis factor ligand superfamily member 2
Uniprot No.	P16599

Application	The quantitative rat TNF (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of TNF, helping to understand its role in immunology.
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 95-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.