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Quantitative Mouse TNF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1129

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative mouse TNF (glycosylated) sandwich ELISA kit is designed to detect mouse tumor necrosis factor (TNF) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 4.052 pg/mL, which can accurately detect low concentrations of TNF in the sample.
Target
TNF
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated TNF-α antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
4.052 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
16 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Tumor necrosis factor
Alternate Names
Tumor necrosis factor; TNF; TNFA; TNFSF2; TNF-α; Tumor necrosis factor ligand superfamily member 2
Uniprot No.
P06804
N-Glycosylation Site
86
O-Glycosylation Site
83
Application Notes
Application
The quantitative mouse TNF (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of TNF, helping to understand its role in immunology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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