Quantitative Mouse TNF (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX1129)

Product Description	The quantitative mouse TNF (glycosylated) sandwich ELISA kit is designed to detect mouse tumor necrosis factor (TNF) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 4.052 pg/mL, which can accurately detect low concentrations of TNF in the sample.
Target	TNF
N-Glycosylation Site	86
O-Glycosylation Site	83
Sample Types	Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	4.052 pg/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	16 pg/mL-1000 pg/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Mouse
Full Name	Tumor necrosis factor
Alternate Names	Tumor necrosis factor; TNF; TNFA; TNFSF2; TNF-α; Tumor necrosis factor ligand superfamily member 2
Uniprot No.	P06804

Application	The quantitative mouse TNF (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of TNF, helping to understand its role in immunology.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated TNF-α antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 90-100%; Plasma sample: n=4, 90-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.