GlycoErase™ CPT2 Knockout HEK293T Cell Line (CAT#: GLJF-0825-JF504)

Description	GlycoErase™ CPT2 knockout HEK293T cell line is a human embryonic kidney (HEK293T) model lacking CPT2 expression. CPT2 resides on the mitochondrial inner membrane and, with CPT1, mediates import and β-oxidation of long-chain fatty acids. In human cells, CPT2 loss provides a tractable system to profile mitochondrial fuel selection, oxidative capacity, and lipid-driven stress responses, aiding studies of FAO deficiencies, metabolic rewiring, and rare disease therapeutics.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	CPT2
Full Name	Carnitine Palmitoyltransferase 2
Alternative Name	CPT1; IIAE4; CPTASE
Location	1p32.3
Gene ID	1376
Summary	This mitochondrial protein works with carnitine palmitoyltransferase I to oxidize long-chain fatty acids. Defects in this gene cause mitochondrial long-chain fatty acid oxidation disorders.