GlycoErase™ CPT2 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF388)

Description	GlycoErase™ CPT2 knockout HEK293 cell line is a human embryonic kidney (HEK293) model lacking CPT2 expression. CPT2 is a mitochondrial protein that, together with CPT1, catalyzes long-chain fatty acid oxidation within the mitochondrial inner membrane. Defects in CPT2 cause fatty acid oxidation disorders. Removal of CPT2 in the HEK293 background provides a human-based system to study mitochondrial fatty acid metabolism, energy homeostasis, and metabolic disease mechanisms, supporting applications in mitochondrial biology, lipid metabolism, and rare disease research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	CPT2
Full Name	Carnitine Palmitoyltransferase 2
Alternative Name	CPT1; IIAE4; CPTASE
Location	1p32.3
Gene ID	1376
Summary	This nuclear protein is transported to the mitochondrial inner membrane, where it cooperates with carnitine palmitoyltransferase I to oxidize long-chain fatty acids. Defects are linked to mitochondrial long-chain fatty acid oxidation disorders.