GlycoErase™ G6PC2 Knockout HEK293 Cell Line (CAT#: GLJF-0825-JF394)

Description	GlycoErase™ G6PC2 knockout HEK293 cell line is a human embryonic kidney (HEK293) model with G6PC2 expression removed. G6PC2 is a member of the glucose-6-phosphatase family expressed in pancreatic islets, and unlike other isoforms, it lacks catalytic activity but serves as an autoantigen in type 1 diabetes. The elimination of G6PC2 in the HEK293 background provides a human-based model for studying autoimmune diabetes, islet biology, and glucose metabolism regulation, enabling research on immune-related mechanisms and novel therapeutic interventions in diabetes.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & Proline & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	G6PC2
Full Name	Glucose-6-Phosphatase Catalytic Subunit 2
Alternative Name	IGRP
Location	2q31.1
Gene ID	57818
Summary	The encoded glucose-6-phosphatase family protein is present in pancreatic islets, lacks phosphohydrolase activity, and is a target of autoimmune attack in diabetes. Multiple splice variants have been described.