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GlycoErase™ GNE Knockout KD Cell Line

CAT#: GLJF-0425-JF691

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Overview Specification Target Information Related Products
Overview
Description
The GNE knockout KD cell line, derived from an extra-cranial rhabdoid tumor, lacks GNE protein, glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase rate-limiting in sialic acid biosynthesis. GNE knockout in rhabdoid cancer cells enables study of sialic acid biosynthesis disruption in this pediatric sarcoma, potentially affecting sialylation of glycans, cell signaling, immune evasion and sialic acid dependent tumor properties relevant to rhabdoid tumor biology.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
KD cell line
Primary Disease
Rhabdoid cancer
Lineage
Soft Tissue
Lineage Subtype
Rhabdoid cancer
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
F-12 & FBS & Penicillin/Streptomycin
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
GNE
Full Name
Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Alternative Name
IBM2, Uae1
Location
9p13.3
Gene ID
10020
Summary
Bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids; rate-limiting enzyme in the sialic acid pathway.
For Research Use Only.
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