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GlycoErase™ HEK293T EOGT Knockout Cell Line

CAT#: GLJF-0425-JF397

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Overview Specification Target Information Related Products
Overview
Description
This EDGT-deficient HEK293T cell line offers a unique opportunity to dissect the role of this O-linked N-acetylglucosamine transferase in modifying extracellular proteins. Researchers can utilize this model to explore the impact of EDGT ablation on protein glycosylation and its downstream effects on cellular processes, including signal transduction and cell-cell interactions.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
HEK293T
Lineage
Embryonic kidney
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Penicillin/Streptomycin
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
EOGT
Full Name
EGF domain specific O-linked N-acetylglucosamine transferase
Alternative Name
C3orf64, AER61, FLJ33770
Location
3p14.1
Gene ID
285203
Summary
Catalyzes the transfer of N-acetylglucosamine to serine/threonine residues of proteins destined for the extracellular environment, a key step in N-linked glycosylation.
For Research Use Only.
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