GlycoErase™ HEK293T POFUT2 Knockout Cell Line (CAT#: GLJF-0425-JF456)

Description	For researchers investigating O-fucosylation of cysteine knot motifs, we are pleased to offer a POFUT2 knockout HEK293T cell line. This cell line lacks the POFUT2-encoded protein O-fucosyltransferase 2, an enzyme that adds O-fucose to serine or threonine residues within cysteine knot motifs in EGF repeats. This model provides a valuable tool for understanding the role of POFUT2 in protein modification and signaling pathways.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	POFUT2
Full Name	Protein O-fucosyltransferase 2
Alternative Name	C21orf80, FUT13, KIAA0958
Location	21q22.3
Gene ID	23275
Summary	Adds O-fucose to serine or threonine residues within cystine knot motifs in EGF-like or thrombospondin type-1 repeats, contributing to protein function.