GlycoErase™ PRKAA2 Knockout Jurkat Cell Line

GlycoErase™ PRKAA2 knockout Jurkat cell line is a Jurkat model lacking PRKAA2 (AMPK α2 catalytic subunit). AMPKα2 governs glucose and lipid metabolism, including regulation of ACC and HMGCR, and contributes to insulin sensitivity and myocardial energy homeostasis. The knockout facilitates studies of glucose control, lipid biosynthesis, and cardiometabolic signaling in mammalian cells.

KO Cell Lines

Human

Suspension-growing

1:3~1:5

Jurkat cell line

T-cell acute lymphoblastic leukemia (T-ALL)

T Lymphocyte

T-cell acute lymphoblastic leukemia (T-ALL)

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

PRKAA2

Protein Kinase Amp-Activated Catalytic Subunit Alpha 2

AMPK; AMPK2; PRKAA; AMPKa2

1p32.2

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.