GlycoErase™ SIGLEC5 Knockout HepG2 Cell Line

GlycoErase™ SIGLEC5 knockout HepG2 cell line is a HepG2 model in which SIGLEC5 expression has been deleted. SIGLEC5 belongs to the CD33-related subset and suppresses the activation of monocytes, macrophages, and neutrophils, and it is also implicated in bacterial immune evasion. The knockout HepG2 background enables investigations into innate immune regulation, host-pathogen interactions, and bacterial immune escape mechanisms, with applications in hepatic infection models, immunology, and therapeutic research.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC5

Sialic Acid Binding Ig Like Lectin 5

CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins have structural motifs in their immunoglobulin-like domains and sialic acid recognition sites in the first Ig V-set domain. The encoded protein, part of the CD33-related Siglec subgroup, inhibits activation in monocytes, macrophages, and neutrophils. It also plays a role in immune evasion by Group B Streptococcus (GBS).