GlycoErase™ SIGLEC5 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF890)

Description	GlycoErase™ SIGLEC5 knockout HepG2 cell line is a HepG2 model in which SIGLEC5 expression has been deleted. SIGLEC5 belongs to the CD33-related subset and suppresses the activation of monocytes, macrophages, and neutrophils, and it is also implicated in bacterial immune evasion. The knockout HepG2 background enables investigations into innate immune regulation, host-pathogen interactions, and bacterial immune escape mechanisms, with applications in hepatic infection models, immunology, and therapeutic research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC5
Full Name	Sialic Acid Binding Ig Like Lectin 5
Alternative Name	CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	8778
Summary	This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins have structural motifs in their immunoglobulin-like domains and sialic acid recognition sites in the first Ig V-set domain. The encoded protein, part of the CD33-related Siglec subgroup, inhibits activation in monocytes, macrophages, and neutrophils. It also plays a role in immune evasion by Group B Streptococcus (GBS).