GlycoErase™ SIGLEC5 Knockout HEK293T Cell Line

GlycoErase™ SIGLEC5 knockout HEK293T cell line is a HEK293T model in which SIGLEC5 expression has been removed. SIGLEC5 belongs to the CD33-related subset and plays a role in suppressing monocyte, macrophage, and neutrophil activation, and is also implicated in bacterial immune evasion. The knockout HEK293T background enables efficient exploration of innate immune regulation and host-pathogen interactions, while supporting applications in high-yield protein expression, lentiviral vector research, and immunology.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC5

Sialic Acid Binding Ig Like Lectin 5

CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.